FastQCFastQC Report
Thu 26 May 2016
SRR1781409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640184
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT8425013.160278919810557No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG256073.999943766167227No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207663.2437549204603675No Hit
ATTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT122111.9074203666445897No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC72351.1301438336478262No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC46030.7190120340402135No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT45880.7166689576746685No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT25810.4031653399647601RNA PCR Primer, Index 43 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT14890.23258938055309097No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG14080.2199367681791485No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC13710.21415717981080437No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT12670.19791185034302639No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT8570.13386776301813227No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT8350.1304312510153331No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG8260.12902540519600614No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC7970.12449545755595266No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGA7400.11559176736688202No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC7310.11418592154755508No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGAATCTCGTATGCCGT6580.10278294990190322No Hit
ATTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT6560.10247053971983054No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC6540.10215812953775788No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAG150.002223901770.004371
CGATCGT150.002223901770.0043770
ACCGATC150.002224590969.998913
AGGCGTA150.002224590969.998940
ACGGGTA150.002224590969.998933
CGCTATA150.002224590969.998917
GACGGCA150.002224590969.998921
TACGTCA150.002224590969.998946
AGGGGGG135650.065.41138570
ATTATAC28250.061.0834581
TCGTAAT650.059.22984340
ACGTAAG650.059.22984340
ACCGAGA252.3904185E-455.99912613
ACGAGCA252.3904185E-455.99912614
CACGTAA900.054.44359639
CGACTAC900.054.44359648
AATGCCG4950.053.73653443
CCCGCAT1900.053.42021632
TCTCGAA4950.053.02947238
ACTCGTA2650.052.8293638
Produced by FastQC (version 0.11.5)