FastQCFastQC Report
Thu 26 May 2016
SRR1781408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436087
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29847868.44459935746767No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG107222.458683703022562No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT10450.23963108278852613No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8830.20248253215528095No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6830.15662012396608932No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5950.13644066436284502No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5900.13529410415811524No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5660.12979061517541224No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5260.12061813353757393No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5200.11924226129189817No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG4910.1125922121044654No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4370.10020936189338366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGT150.002215971270.0636543
GCTCGCG150.002215971270.0636542
TAGGTCG150.002216981370.055639
CATTCTG150.002216981370.055639
AAACGGT150.002216981370.055644
TCCATCA150.002219002270.0395135
CTGAGCC150.002219002270.0395147
GTCCATC150.002219002270.0395134
ACAGTTA150.002220013270.0314753
TCCGATA150.002220013270.0314759
CAGTTAT150.002220013270.0314754
TTCAATT150.002220013270.0314750
CCGTCTC150.002220013270.0314755
GCAATCG150.002220013270.0314761
TCGAGCC150.002220013270.0314765
TCAATTT150.002221024670.0234251
AATCGAG150.002221024670.0234263
TTCGATG150.002225073569.9912830
TCTTATG150.002227169.975221
CTGATAC353.7161954E-969.975211
Produced by FastQC (version 0.11.5)