FastQCFastQC Report
Thu 26 May 2016
SRR1781408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436087
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT17269939.601960159326005No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4366410.012680955864312No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT200724.60275128586727No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189264.339959686943202No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC146313.355064471080312No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC82031.8810466718796937No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT41880.9603588274816723RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT29400.6741774003811166No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC24830.5693817976688138No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT22330.5120537874323243No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT20580.4719241802667817No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG17660.4049650643105619No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC15030.34465599754177495No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT14750.3382352603952881No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC14130.3240179138566387No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG11130.25522430157285125No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT10160.23298103360109337No Hit
CTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT9850.22587236033176863No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC9670.2217447435947414No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC9630.2208274954309576No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT9430.21624125461203844No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9200.21096707767028136No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGA8290.19009968194419918No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAGT7720.1770288956102796No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTAAGCCGT7700.1765702715283877No Hit
CTTATACACATCTCCGAGACCACGAGACGACTTAGTATCTCGTATGCCGT7410.1699202223409549No Hit
TCTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT7380.16923228621811703No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGAATGCCGT6890.15799599621176508No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5950.13644066436284502No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTTAGTATCTCGTATGCCGT5710.13093717538014202No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5670.1300199272163582No Hit
CTTATACACATCACCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5190.11901294925095221No Hit
CTTATACACATCTCAGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT5080.11649051680054669No Hit
CTTATACACATCTCCGAGCACACGAGACGACTTAGTATCTCGTATGCCGT4960.11373877230919519No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGACGT4850.11121633985878965No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCAG4510.10341973046662707No Hit
TCTTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCG4480.1027317943437892No Hit
ATTATACACATCTCCGAGCCAACGAGACGACTTAGTATCTCGTATGCCGT4400.10089729801622153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG150.0022198670.032770
GCACAGT150.0022198670.032766
ATAAAGG353.6925485E-970.032766
AATAGCG207.904798E-570.032769
AGAGGCG150.0022198670.032768
AATGAGT150.002223903570.000576
GGCATTC150.002223903570.000572
ATTTAGT150.002224915469.99254632
TCCATGC150.002224915469.99254649
AAATCGA207.927287E-569.99254637
GATGTGG150.002224915469.99254610
CAAGTTG150.002224915469.99254629
CGTATCT150.002224915469.99254634
CGTATCA252.8398317E-669.99254641
AGTCAAC150.002224915469.99254648
GGATGTG150.002224915469.9925469
TCCGATC252.8398317E-669.99254613
CCGATCC301.02354534E-769.99254614
ACTGAAT252.8398317E-669.99254653
TCCGACC301.02354534E-769.99254613
Produced by FastQC (version 0.11.5)