FastQCFastQC Report
Thu 26 May 2016
SRR1781407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204028
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34944429.02291308839994No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG142891.1867664207144686No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT14180.11777134751019078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTC150.002229742269.9618763
TTGTACG501.4551915E-1162.976152
TATGCGG452.7153874E-854.4147953
CGGATAG200.006962533652.48012518
CTTGCGA200.006962533652.48012512
TGTGCGG1000.052.4714053
TTGTGCG1950.052.0315782
CGTATCA25350.051.9259156
CCGTATC25050.051.43187755
TCGCCGT25600.051.42310352
CGCCGTA25500.051.2128653
GTGGTCG28800.050.93683248
CGGTGGT29300.050.91040446
TGTACGC552.2973836E-950.8813673
GTCGCCG27550.050.70627251
GGTCGCC27550.050.70416350
GCCGAAT900.050.57002354
TGGTCGC27700.050.42749449
TCTCGGT26300.050.341643
CTCGGTG27200.050.21279544
Produced by FastQC (version 0.11.5)