FastQCFastQC Report
Thu 26 May 2016
SRR1781407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204028
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT20376916.92394196812699No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG486104.037281525014368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240001.9933091257013955No Hit
ATTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT217981.8104230134182928No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC182191.513170790048072No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC89930.7469095403096938No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT43310.35970924264219767RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT27710.23014414947160697No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC25200.20929745819864656No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT23590.19592567614706632No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT21140.17557731215553127No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC19670.1633682937606102No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT19120.15880029368087786No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG17230.14310298431597937No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT13990.11619331111901053No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG13870.11519665655615981No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT13760.11428305654021335No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC12640.10498094728694017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG150.002223930370.00793568
GCGTTAC150.002224296870.0050357
GCCGATA150.00222539669.9963147
AGGGGGG289050.065.8905370
ATTATAC45300.060.508571
CGTATCC800.056.87199841
CCGTCTA6850.055.1795747
CCGACTT5700.054.6462447
AAGCCGT3600.053.469444
TGCCGAC6850.053.1358845
GTACCGG200.00695242652.49941332
CTTACGT200.00695242652.49941330
ACTTGCG200.00695356852.49722727
GAATCGT200.00695356852.49722737
AGATTCG200.00695356852.49722718
ATGACGT8400.052.08058544
AAGGGGG368900.051.495469
ATGCCGA7350.051.42585844
CGACTTC6150.051.2168148
TCGTAAG5150.050.96818540
Produced by FastQC (version 0.11.5)