FastQCFastQC Report
Thu 26 May 2016
SRR1781406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436116
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26204360.08561942235552No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG94632.1698355483403495No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT9180.210494455603555No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5870.13459721725412505No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5700.13069917177998513No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5630.1290940942318099No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4750.10891597648332095No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.10501793100918104No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4450.10203707270542699No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG4430.10157847912023407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACATT150.00222079570.0252542
TTAGATA150.00222079570.0252537
ACCAGTC150.00222079570.0252548
CAGTCCA150.00222079570.0252550
GTGCCCC150.00222079570.0252549
AACGTCT150.002221806470.0172264
CGTGTGC252.8396898E-669.993121
CTTGGAC150.002224842869.993111
CTTATGC401.3460522E-1069.993111
CTTAGAC207.926962E-569.993111
GACCGCC207.935978E-569.9770627
CTAAGTG252.8435643E-669.9770630
GACGGAC252.8435643E-669.9770623
GCCCAAG150.002226868669.9770528
GCAGTTA150.002226868669.9770515
CGACTTG150.002226868669.9770515
ACGAGAT150.002226868669.9770524
TACGTCG150.002226868669.9770519
ACACGTC207.9450016E-569.9610066
ACATGTG150.00222889669.9610068
Produced by FastQC (version 0.11.5)