FastQCFastQC Report
Thu 26 May 2016
SRR1781406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436116
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT14658233.610782452375055No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG404569.276431041282594No Hit
ATTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT192274.408689431252236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163213.7423529519669074No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC124252.8490126480110796No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC68931.5805427913674346No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT36470.8362454025993085Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT23210.5321978556163957No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC18650.42763851819240756No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTAATCTCGTATGCCGT16060.36825064890992304No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT14330.3285823037907346No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG13420.30771629566445624No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG12060.276531931871337No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC11510.2639206082785314No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC10560.2421374129818672No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGACTTATCTCGTATGCCGT9990.2290674958038687No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9710.22264718561116767No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGCCTTATCTCGTATGCCGT9660.22150070164818533No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT9100.20866008126278332No Hit
CTTATACACATCTCCGAGCCAACGAGACTGTGCCTTATCTCGTATGCCGT7380.16922103293619128No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCATATCTCGTATGCCGT7170.16440580029166554No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT7120.16325931632868318No Hit
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC7090.1625714259508938No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGA7080.16234212915829735No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGACGT6910.1584440836841574No Hit
CTTATACACATCTCCGAGACCACGAGACTGTGCCTTATCTCGTATGCCGT6250.14331049537279072No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTAAGCCGT6090.1396417466912473No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCAGT5930.13597299800970383No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTAACTCGTATGCCGT5900.13528510763191445No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGAATGCCGT5810.13322143649854626No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5460.12519604875767No Hit
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT5140.1178585513945831No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTAATCTCGTATGCCGT4440.10180777591283054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCA401.3460522E-1070.0026456
GCACATC207.921628E-570.002646
GTCCGGG150.002223643270.002641
ATTTAGG150.00222465569.9946142
TACCCCG150.00222465569.9946140
CCGTCCG150.00222465569.9946155
ACGGAAT150.00222465569.9946144
TAGATCG150.00222465569.9946117
AGTTACG150.00222465569.9946140
TTACGGA150.00222465569.9946142
GCGGATA150.00222465569.9946115
GCTAAGC150.00222465569.9946155
GTGACCT150.00222465569.9946118
GCGTATC150.00222465569.9946111
AAAGACG207.926129E-569.9946143
TCAGTCT207.926129E-569.9946146
TAAGCCC252.8393333E-669.99460643
GCCCCGA150.00222566769.9865951
ACGCCAG150.00222566769.9865923
ATTCGCT150.00222566769.9865938
Produced by FastQC (version 0.11.5)