FastQCFastQC Report
Thu 26 May 2016
SRR1781405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477807
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26908656.31688108378488No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG109392.2894181123340593No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT11020.23063705638469087No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9370.19610428478444222No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6300.1318524006554948No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6280.1314338216057948No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5760.12055076631359524No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5370.11238847484444556No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG5320.11134202722019561No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5100.10673765767349579No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTGG150.002222600670.0119558
ACCGGGA207.933629E-569.9826220
TAACCCG150.002226296869.9826217
ATACCGT150.002229072269.960655
GTATACG150.002231849869.938682
ACGCGTC750.065.29666
TATGCGC550.063.587273
TGGCCGT453.8380676E-1062.2458852
CGCGTGT401.0701115E-861.2155657
TATACGC1000.059.45413
TTGTGCG1950.059.178882
GGGCGTA308.35896E-658.349453
GTTAGAC308.400579E-658.3005371
TTAGACG308.416231E-658.2822342
TGCGCGT1450.057.898465
CGCGTCT2300.057.7935757
TCTGGCG3650.057.50791511
CGACGAG1100.057.24651722
ATGTCGG509.731593E-1056.04478542
CGGTATC252.3863671E-456.01542355