SRR1781405_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781405_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477807
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	148115	30.998917973156527	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG	38053	7.964094289116735	No Hit
ATTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	19796	4.143095433930436	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	18761	3.9264807757106954	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC	14380	3.009583367342881	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC	6828	1.4290288756757437	No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT	3808	0.7969745106287686	RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT	2710	0.5671746123434777	No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC	2076	0.43448505358858286	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT	1812	0.379232619028185	No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	1682	0.35202498079768607	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC	1529	0.32000368349563735	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT	1454	0.304306969131888	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG	1326	0.27751790995108905	No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC	1210	0.25324032506849	No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC	1102	0.23063705638469087	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT	1093	0.22875345066104097	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT	1046	0.21891684299309133	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC	1036	0.2168239477445914	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG	1003	0.2099173934245417	No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT	821	0.1718266999018432	No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC	789	0.1651294351066435	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGA	789	0.1651294351066435	No Hit
CTTATACACATCTCCGAGCCAACGAGACCAATCTTAATCTCGTATGCCGT	770	0.16115293413449364	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAAACTCGTATGCCGT	741	0.15508353791384388	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGACGT	726	0.151944195041094	No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTCTTAATCTCGTATGCCGT	651	0.13624748067734463	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGAATGCCGT	648	0.13561961210279463	No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG	629	0.1316431111306448	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCAGT	607	0.127038741583945	No Hit
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT	600	0.12557371490999503	No Hit
CTTATACACATCTCCGAGACCACGAGACCAATCTTAATCTCGTATGCCGT	555	0.11615568629174541	No Hit
ATTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT	533	0.11155131674504559	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCATAATCTCGTATGCCGT	510	0.10673765767349579	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGT	15	0.002224634	69.99582	15
CTACGAT	15	0.002224634	69.99582	12
TATCGAT	15	0.002224634	69.99582	31
ATCGCTC	15	0.002224634	69.99582	55
TCCATCG	20	7.930339E-5	69.988495	51
GTTCGTA	15	0.0022255573	69.98849	25
AACGATA	15	0.0022255573	69.98849	21
ACGACGT	15	0.0022255573	69.98849	45
CCCGATT	15	0.0022255573	69.98849	8
ATTATAC	3550	0.0	67.14385	1
AGGGGGG	21535	0.0	65.479614	70
CGTCTAA	60	0.0	64.15611	48
TCTTTAT	860	0.0	62.677235	1
CATAATC	815	0.0	61.407368	33
ACGATAC	40	1.0668373E-8	61.23993	22
CGATACC	40	1.0668373E-8	61.23993	23
ATCATAA	855	0.0	60.17184	31
TCGTATC	35	2.9756484E-7	59.990135	40
ATAATCT	790	0.0	59.363537	34
ACACGTA	65	0.0	59.22103	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content