FastQCFastQC Report
Thu 26 May 2016
SRR1781404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282697
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20130271.20768879754648No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG81912.8974485049363805No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT8950.31659338443633994No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5110.18075890441002204No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG4600.16271838753152668No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.1598885025309784No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4080.14432413502796282No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3920.13866436502686622No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3610.12769856064974158No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440.12168505502357647No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.11602528502247991No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3080.10895057252110917No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG2830.10010718189439577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATT150.002217057970.0485438
CAATGAA150.002217057970.0485438
AGCTCTC150.002217057970.0485440
TGCGATC150.002217057970.0485437
CTCTCGG150.002217057970.0485442
TAGCTCT150.002217057970.0485439
ACTTCGT150.002217057970.0485442
ACATACT150.002217057970.0485446
GTGACTT150.002217057970.0485439
ATATGCA150.002217057970.0485443
AGTTGAT150.002217057970.0485437
ATGCATA150.002217057970.0485445
ATCATAT150.002217057970.0485440
GCTCTCG150.002217057970.0485441
ATCGATC150.002217057970.0485441
CATATGC150.002217057970.0485442
TCATATG150.002217057970.0485441
GTAGCTC150.002217057970.0485438
AACATAC150.002217057970.0485445
GTCAATG150.002218616470.0361336
Produced by FastQC (version 0.11.5)