FastQCFastQC Report
Thu 26 May 2016
SRR1781404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282697
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT11547940.8490362472895No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG3008710.64284375143705No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT148845.265001043520094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143505.076106219733496No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC108643.842983830744578No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC45941.6250614615648558No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT25440.8999034301743564RNA PCR Primer, Index 45 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT18430.6519347570013123No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT14250.5040732657226642No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC13850.4899238407199228No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT12690.44889050821197257No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT9830.34772211944237114No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG9290.3286203956886702No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG8560.30279769505866705No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC8370.29607671818236486No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC8340.29501551130715925No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7700.27237643130277295No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTATCTCGTATGCCGTC6720.23771034004605637No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACATTTATCTCGTATGCCGT6700.23700286879591934No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT5740.20304424878933983RNA PCR Primer, Index 45 (95% over 22bp)
CTTATACACATCTCCGAGCCAACGAGACGGTCATTTATCTCGTATGCCGT5570.1970307431631747No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGACGT5290.18712614566125568No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCAGT4970.17580660565906253No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGA4960.17545287003399399No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC4860.17191551378330863RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGACCACGAGACGGTCATTTATCTCGTATGCCGT4540.16059597378111548No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG4370.15458246815495036No Hit
TCTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT4330.1531675256546762No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGAATGCCGT4140.146446548778374No Hit
CTTATACACATCTCCGAGCACACGAGACGGTCATTTATCTCGTATGCCGT3720.1315896525254955No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTAACTCGTATGCCGT3540.12522241127426184No Hit
CTTATACACATCACCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT3440.12168505502357647No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT3430.12133131939850794No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGACGT3390.1199163768982338No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT3390.1199163768982338No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAATTTATCTCGTATGCCGT3370.11920890564809672No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT3190.11284166439686308No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTAAGCCGT3110.11001177939631479No Hit
CTTATACACATCTCAGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT3030.10718189439576649No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCAGT3010.10647442314562942No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCAG2860.10116838876960137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG401.3460522E-1070.0309870
AGGGGGC207.900951E-570.0309870
AAACTGG150.00221926570.03097567
ACTCGGC150.00221926570.03097569
AGTGGGG353.6870915E-970.03097570
GATAAAA150.00221926570.03097560
CCCATAC150.00221926570.03097560
ATGTGTG150.00221926570.03097570
AGGTGGG455.456968E-1270.03097570
GCCGTTA150.00221926570.03097559
GTTATTC150.00221926570.03097562
GGGTGGA150.00221926570.03097563
CAGCTCC150.002222383670.0061954
TTCATTG150.002222383670.00619534
ACTCGGT150.002222383670.00619523
ACATGAA150.002222383670.00619556
GATACAC150.002222383670.0061953
CCACGAA150.002222383670.00619520
GGTCTAT150.002222383670.00619529
GAAGTCA150.002222383670.00619522
Produced by FastQC (version 0.11.5)