FastQCFastQC Report
Thu 26 May 2016
SRR1781403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368842
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30017881.38389879677477No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG27220.7379853704296149No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7330.19873007954625557No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5440.14748862656638886No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5050.13691499341181318No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4780.12959478584326078No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4540.12308793467121423No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4020.10898975713178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA150.00221975670.0313638
GAGGTCT150.00221975670.0313639
ACTGCAA150.00221975670.0313640
ATCTAGG252.8296254E-670.0313642
AGAGCTC301.01919795E-770.0313640
GGTAGGT252.8296254E-670.0313637
GGTAGAT252.8296254E-670.0313637
GACTGCA150.00221975670.0313639
CTCGGAG150.00221975670.0313644
ACAAGGG150.00221975670.0313643
GAGAGCT150.00221975670.0313639
TAGATAT150.00221975670.0313639
TCTAGGT207.903933E-570.0313543
GGATCTC207.903933E-570.0313540
AGGTGTC207.903933E-570.0313540
ATGTCGG207.903933E-570.0313542
CATGAGA150.002220951370.0218561
TCAGGAG150.002220951370.0218560
CAGTAAA150.002220951370.0218561
CGTAGCA207.909248E-570.0218556
Produced by FastQC (version 0.11.5)