FastQCFastQC Report
Thu 26 May 2016
SRR1781403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368842
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT20887756.630481344315456No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG197555.355951870990831No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC123183.3396413640529006No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT121663.298431306629939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42781.1598462214173006No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC28770.7800087842490823No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT24650.6683078391289495TruSeq Adapter, Index 18 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT22450.6086617033851893No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT21370.5793808731109797No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT21080.5715184279447568No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC16590.44978608726771896No Hit
ATTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT11150.3022974607013301No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG10410.28223466958751986No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8680.23533111738901752No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8470.22963762261347678No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8350.22638419702745347No Hit
CTTATACACATCTCCGAGCCCACGAGACTACAACTCATCTCGTATGCCGT7970.21608168267171307No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAACCTCATCTCGTATGCCGT7740.20984595029850178No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT7380.20008567354043194No Hit
CTTATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT7130.1933077035695501No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCCTCATCTCGTATGCCGT7050.19113875317886792No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT6920.18761420879400936No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT6860.18598749600099773No Hit
CTTATACACATCTCCGAGACCACGAGACTACACCTCATCTCGTATGCCGT6530.17704057563943368No Hit
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC6490.1759561004440926No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC5740.15562219053144707No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG5440.14748862656638886No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGACGT5210.14125289419317757No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCAGT4780.12959478584326078No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCACATCTCGTATGCCGT4740.1285103106479197No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT4550.12335905347004951No Hit
CTTATACACATCTCCGAGCACACGAGACTACACCTCATCTCGTATGCCGT4340.11766555869450876No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTAATCTCGTATGCCGT4330.1173944398956735No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTAAGCCGT4260.11549660830382656No Hit
CTTATACACATCTCCGAGCCCACGAGACAACACCTCATCTCGTATGCCGT3940.10682080674109783No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT3940.10682080674109783No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3750.1016695495632276No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGAATGCCGT3750.1016695495632276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA800.070.0174941
AATTATA301.0205986E-770.0174941
ACTTTAT207.911691E-570.0174941
ATTATAA150.002221499870.0174941
CTAAATA150.002221499870.0174941
TCTCTGG150.002221499870.0174941
AAGGGGC353.7016434E-970.00869
GAAGGGG650.070.00799668
CTCTGGG150.002223890769.998512
TTGTCCT150.002223890769.9985117
TCCTCAT150.002223890769.9985132
ACACGAA150.002223890769.9985138
AAACCAG150.002223890769.9985120
CATGACA150.002223890769.9985157
TCCCACG301.0225085E-769.9985118
GCCTTCT301.0225085E-769.9985146
TATACGC150.002223890769.998513
TATACGA150.002223890769.9985111
ACTGATG150.002223890769.9985127
CGCCCTT150.002223890769.9985112
Produced by FastQC (version 0.11.5)