FastQCFastQC Report
Thu 26 May 2016
SRR1781402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755734
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9910913.114270364969686No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG27020.35753320612808215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC4300.055.3608355
CGCCGTA4500.055.23052253
CGTATCA4650.052.69958556
CGATTTT200.00693426352.5322440
GTTACCG200.006945167752.5113734
TTATGCG200.006952444652.497472
CGACGTA200.006961548252.48010318
TACCGGG200.006963370452.476634
TCGCCGT4550.052.31902752
GTCGCCG4800.051.78204351
GTGGTCG5000.051.1144548
ATCTCGG4800.050.34339542
TCGGTGG5450.050.11586445
GGTCGCC5000.049.71076250
GATCTCG4900.049.3159841
CGGTGGT5400.049.2829846
GCCGTAT4950.048.79521654
CTCGGTG5150.048.27566544
TCTCGGT5050.047.15765443
GTTCGCC305.8871927E-446.6520629
Produced by FastQC (version 0.11.5)