FastQCFastQC Report
Thu 26 May 2016
SRR1781402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755734
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT438485.802041459031882No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG144701.9146948529509058No Hit
ATTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT83921.1104436216975815RNA PCR Primer, Index 47 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55440.7335914488431114No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC33920.44883517216375074No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT31280.41390224602836445RNA PCR Primer, Index 47 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC31020.4104618820907886No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT23110.3057954253745365Illumina PCR Primer Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC11010.14568618058734953RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG8280.10956235924280235No Hit
ATTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT7700.10188770122820992No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC150.002224625669.9998633
AGGGGGG80100.061.6103770
GGCCGAT252.3905344E-455.9998932
CTACTCG200.006951172452.49998
CGAGTAC200.006951172452.499948
AGACGCG200.006951172452.499963
ACCGTGT200.006951172452.499946
CACGTTA200.006951172452.499964
CACGTAT1600.052.499939
CGAAAGC608.367351E-1152.49989741
ACGCAGA1750.051.999931
ATTATAC19350.051.5571141
TCGTAAG2550.049.41166740
ACCTCGC1450.048.2757728
CGAAGAT1700.047.35285632
CCGTCTA3000.046.6665847
CTCGAAG1500.046.6665830
CTCATCG604.9367372E-946.66657667
TGCGTGA305.878174E-446.66657663
AAGGGGG106100.046.61159569
Produced by FastQC (version 0.11.5)