FastQCFastQC Report
Thu 26 May 2016
SRR1781401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556601
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18045032.41999205894348No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG72751.3070404113539142No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT7470.13420744842355656No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6000.10779714732815787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATT252.8456325E-669.9727725
CACGAAC150.002227733869.9727723
CGCGTCT1050.063.2973257
ATGCGCG501.4551915E-1162.9641844
TACACGT509.858923E-1055.9681635
TGCGCGT1150.054.7514655
GCCGAAT653.6379788E-1253.86393454
CGCCGTA14950.053.86393453
CGTCTGG2150.053.6903889
CCGTATC14950.053.39555455
CGTATCA15350.053.36786356
TCTGGCG3150.053.30301311
GCCGTAT15150.053.1528654
ATCTCGG15100.053.1113242
TCGCCGT14900.053.10477452
GATCTCG15350.052.70261441
TCGTCGG200.00693862552.52205347
GCGTCGA200.00694109352.5173341
TTGTACG200.006955915652.4890142
CGTCGAT200.006955915652.4890142
Produced by FastQC (version 0.11.5)