FastQCFastQC Report
Thu 26 May 2016
SRR1781401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556601
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT9191916.51434330876157No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG263354.7313964581450625No Hit
ATTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT150742.708223664707753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132522.38087966065458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC79401.4265155829759557No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC51990.934062281598488No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT30950.556053618301081Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACGCCAAATCTCGTATGCCGT18320.3291406231753087No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC15440.2773979924577929No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT11540.2073298466944903No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGCCATATCTCGTATGCCGT11230.2017603274158688No Hit
CTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG9390.16870253556856707No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTAAGCCGT9050.1625940305533048No Hit
TTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC8930.16043808760674164No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC8510.15289228729377058No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGCCATATCTCGTATGCCGT8410.15109566817163464No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC8090.14534648698079955No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCAGT7540.13546508180905173No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGACATATCTCGTATGCCGT7470.13420744842355656No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCAATATCTCGTATGCCGT7380.1325904912136342No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT7190.12917691488157584No Hit
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC6720.12073280500753683No Hit
CCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG6320.11354632851899296No Hit
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGA6230.1119293713090706No Hit
CTTATACACATCTCCGAGCCAACGAGACAACGCCATATCTCGTATGCCGT5580.10025134701518681No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT150.002224401669.9991923
AGGGGGG150100.064.7352570
ATTATAC30550.062.558091
CGTCATA401.0666554E-861.2437948
ACTCGAA650.059.22476638
AAGACGA509.822543E-1055.9943244
CCCGTCT252.39116E-455.9943246
ATACCGT252.39116E-455.9943244
AACTCGA700.054.99442737
CCGACTT3300.053.0249347
CGACTTC3500.052.99462548
TCTTTAT8500.052.710011
AATGCCG4250.052.70054243
TTTATAC9300.052.6922953
AGCGACC200.006948009652.5041125
GACGGAC200.006948009652.50411270
CGGGTTA200.00695047952.49939714
TATCCGG200.00695047952.49939727
CTTCGGT200.00695294952.4946811
CCATCGA200.00695294952.4946836
Produced by FastQC (version 0.11.5)