FastQCFastQC Report
Thu 26 May 2016
SRR1781400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320889
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18825558.6667040627756No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG64402.0069245128377724No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT6210.19352486373792807No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5430.1692173929302656No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4500.14023540850574498No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4470.13930050578237335No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4050.12621186765517048No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3920.12216062252056008No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3450.10751381318773781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACT150.0022130570.0826744
TAGATCG150.0022130570.0826739
CGACTCC207.873728E-570.0826736
ATCGACT150.002215794870.06077634
CGCGGTA207.892032E-570.04983553
TTAGCGG150.002218542470.0388967
GTTAGCG150.002218542470.0388966
CGGCCTT150.002230937869.9405825
GCCTCCC207.953291E-569.9405825
GGCTCTT207.953291E-569.9405827
CGTCTGA150.002232318369.929679
GTCAAAT150.002232318369.9296722
GGTGCTT207.959438E-569.9296711
ACGTTTT150.00223508169.9078757
CACGTCT207.971743E-569.9078757
ACGCGTC252.8585928E-669.9078756
TTATGCG207.9779005E-569.896982
TTGTACG150.002236463469.896982
TTGTGCG700.069.896972
CGCGTCT750.065.2473457
Produced by FastQC (version 0.11.5)