FastQCFastQC Report
Thu 26 May 2016
SRR1781400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320889
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT9878330.784165240940016No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG274008.53877820679425No Hit
ATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT152894.764575912542966No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118273.6856981697721016No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC86472.694701282998171No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC49221.533863734811726No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT28570.8903390268909187RNA PCR Primer, Index 20 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC16630.5182477429890087No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT13730.4278738130630841No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT13130.4091757585956515No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT12460.38829626444035165No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT12070.3761425290365204No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG9940.30976443567713446No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC9150.2851453306283481No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC8910.27766610884137505No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC8280.2580331516505707No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA7280.226869727538183No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG7050.2197021399923338No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTAAGCCGT7020.21876723726896216No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT6770.21097638124086524No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT6710.20910657579412192No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGAATGCCGT6380.198822645837034No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT6170.19227832677343257No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC6050.18853871587994603No Hit
CTTATACACATCTCCGAGCCAACGAGACGAAATGCCATCTCGTATGCCGT5640.17576171199386706No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCAGT4790.14927280149833744No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCACGTATGCCGT4750.1480262645338419No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4660.14522155636372702No Hit
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT4420.13774233457675397No Hit
CTTATACACATCTCCGAGACCACGAGACGAAATGCCATCTCGTATGCCGT4260.1327561867187719No Hit
ATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT3910.12184898827943619No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGACATCTCGTATGCCGT3650.11374649801021537No Hit
TCCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC3490.10876035015223333No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCAACTCGTATGCCGT3360.10470910501762293No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATATCGTATGCCGT3350.10439747077649902No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCG3260.10159276260638414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTG150.002215593470.062465
CGGCCTA150.002216964770.05147660
ATGACGG150.002218336870.0405559
CCGCTAA150.002221082870.018711
GATTCAA150.002222456770.0077956
GTAGACT150.002223831169.996883
CGCTAAC150.002223831169.996882
CGGTGTT150.002223831169.996884
GACTGTT150.002223831169.996886
GCTAACC150.002223831169.996883
GGTAGAC207.921668E-569.996882
CCCGACC150.002223831169.996883
TCCGGAG150.002225206569.9859826
ACTATTG150.002225206569.9859837
TCGCATT150.002225206569.9859852
ACGCCTT150.002225206569.985989
CTCCGAC252.8397026E-669.9859812
CTTCCCC150.002225206569.9859813
TAGACTA150.002225206569.9859824
TCCGACC150.002225206569.9859813
Produced by FastQC (version 0.11.5)