FastQCFastQC Report
Thu 26 May 2016
SRR1781399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418208
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29589770.75354847348689No Hit
CTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGTG118612.8361485193970464No Hit
TCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGGT12440.29745963730966407No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10800.2582447012013161No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7930.1896185630117071No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7050.16857640217308134No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6620.15829443721784375No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6220.14872981865483204No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5530.13223085163363685No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5510.13175262070548627No Hit
CTCTTATACACATCTGACGCTGCCGACGAACCGCCCAGTGTAGATCTCGG5360.12816588874435686No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4380.1047325732649782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAG252.8268441E-670.045721
GTGATGG252.8268441E-670.045721
CTTGTAC550.070.0457151
GTTATAC455.456968E-1270.0457151
AATTATA301.0180702E-770.045711
CTTATCA150.002218160370.045711
TATTAGG150.002218160370.045711
GTTATGC150.002218160370.045711
TCACGGT401.3460522E-1070.0289643
TAGGCGA207.9065125E-570.0289641
TGGCAAA150.00222026770.0289539
TAGAGCG150.00222026770.0289539
GTGGATC150.00222026770.0289538
AATGGCA150.002221320870.0205837
AAGGTAG207.9112E-570.0205837
ACGTATC252.8348932E-670.0122155
CGGGTCA252.8348932E-670.0122156
GACGTAT252.8348932E-670.0122154
AGCCGTA252.8348932E-670.0122153
TTAGACA353.7016434E-970.01222
Produced by FastQC (version 0.11.5)