FastQCFastQC Report
Thu 26 May 2016
SRR1781399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418208
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT16907340.42796885760196No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG4208610.063413421072767No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT212745.086942382737777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207944.972166959981636No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC138763.3179661795087614No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC76761.8354503022419466No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT43961.0511515800749867No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT33190.7936242252658965No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC27100.6480029076440431No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT22390.5353795240645803No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT19930.4765571199020583No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT19910.4760788889739077No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG16470.393823169332007No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC14880.35580381054403554No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC14730.3522170785829061No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG11350.27139605172545717No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC10990.26278789501874666No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC10260.24533246614125026No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGACGT9940.23768077129084092No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT9610.22978996097635626No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGA9160.21902976509296807No Hit
CTTATACACATCTCCGAGCCAACGAGACCAAGAATTATCTCGTATGCCGT8310.1987049506465682No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTAAGCCGT8050.1924879485806106No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCAGT7790.186270946514653No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG7780.1860318310505777No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGAATGCCGT7420.17742367434386716No Hit
CTTATACACATCTCCGAGACCACGAGACCAAGAATTATCTCGTATGCCGT7150.17096755681383427No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTAACTCGTATGCCGT7150.17096755681383427No Hit
TCTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT6470.15470770525671435No Hit
TCCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC5640.13486112173846507No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATAATCTCGTATGCCGT5590.13366554441808862No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT5490.1312743897773357No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT5390.12888323513658276No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGACGT5330.127448542352131No Hit
CTTATACACATCTCAGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT5120.12242711760654984No Hit
CTTATACACATCACCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT4830.11549276914836636No Hit
CTTATACACATCTCCGAGCACACGAGACCAAGAATTATCTCGTATGCCGT4810.11501453822021578No Hit
ATTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCAGT4660.1114278062590864No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCAG4550.10879753615425816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTAT150.002221156470.021891
GTATGGG207.9104684E-570.021891
ATGATAC150.002221156470.021891
CTTACAC207.9104684E-570.021891
CTTATAT150.002221156470.021891
ATTTACA150.002221156470.021891
GTTTGCA150.002222210270.0135165
ACTGAAA207.915156E-570.0135159
TATGTTT150.002222210270.0135162
GCTGGTC150.002222210270.0135165
TGTTTGC150.002222210270.0135164
CACTGCT150.002222210270.0135161
CTGAAAT207.915156E-570.0135160
AGAAACA150.002222210270.0135159
TCCACTG150.002222210270.0135159
ATGTTTG150.002222210270.0135163
ATCGCTG207.915156E-570.0135165
TTATGTT150.002222210270.0135161
CGCTGGG207.9198464E-570.0051467
AGGGGCG550.070.0051470
Produced by FastQC (version 0.11.5)