FastQCFastQC Report
Thu 26 May 2016
SRR1781398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274538
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20440274.45308117637632No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG21800.7940612957040556No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7870.2866634127151797No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6340.23093342269558315No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5570.202886303535394No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4390.15990500404315613No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3940.14351383050798067No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3780.13768585769547384No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440.12530141546889684No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3100.11291697324231982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC207.9092075E-570.0154951
TAGGTCT252.8315044E-670.01549539
ATTGGGA150.002221144770.015491
ATGGCCG150.002221144770.0154946
TGTGGAT150.002221144770.0154937
GTAGGGC150.002221144770.0154938
CATGGCC150.002221144770.0154945
GTTATGC150.002221144770.015491
TTATGGA150.00222275170.002732
TTATGCA150.00222275170.002732
GAGCGGT150.00222275170.0027358
CCGGAGC150.00222275170.0027355
AATCCGT150.00222275170.0027363
AGGATTA150.00222275170.0027359
GGTCTCT150.00222275170.0027329
GTATCAG150.00222275170.0027357
GGATTAA207.916351E-570.0027360
CAATCCG150.00222275170.0027362
TTGGGAA150.00222275170.002732
CTGTGTA150.00222275170.0027334
Produced by FastQC (version 0.11.5)