FastQCFastQC Report
Thu 26 May 2016
SRR1781398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274538
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT8653731.520955204743974No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG195757.130160487801325No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT139675.087456017017681No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT105663.8486475460591976No Hit
CTAAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT51121.8620373135959318No Hit
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT47211.7196162279902962No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC43541.585937101603421No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38631.4070911859196176No Hit
CAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT29301.0672475212903132No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG27821.013338772774625No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT25780.9390321194151628RNA PCR Primer, Index 17 (95% over 22bp)
CATAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT25200.9179057179698257No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC24610.8964150682237068No Hit
TCTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG22980.8370425951962934No Hit
CAAAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT18190.6625676591218702No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAACAGATCTCGTATGCCGT12840.46769481820367304No Hit
TCAAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG11060.40285862066453454No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC9880.3598773211722967No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC7340.2673582527737508No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC6870.250238582637012No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTACAGATCTCGTATGCCGT6510.23712564380887163No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT6170.2247412015822946No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5890.21454224916040765No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATAAAGATCTCGTATGCCGT5400.1966940824221055No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT5130.18685937830100025RNA PCR Primer, Index 17 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCG4910.17884591568380334No Hit
CTCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC4840.1762961775783316No Hit
CTCTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC4620.1682827149611347No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG4480.16318323875019122No Hit
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC4090.14897755501970583No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG3660.1333148780860937No Hit
CTAATACACAACTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3550.12930814677749528No Hit
TCTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT3090.11255272494153815No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGAATGCCGT2970.10818174533215802No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGCTCTCGTATGCCGT2830.10308226912121454No Hit
CTAATACACATCACCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT2830.10308226912121454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGAG207.913497E-570.00783570
AAACTGG150.00222210970.00783568
TCCTTAT550.070.0078351
AAACCCA252.833347E-670.0078354
CTTTACA550.070.0078351
ACGCATC207.913497E-570.0078356
ATGGTGA150.00222210970.0078356
AATTAAA150.00222210970.00783557
TCAATAT207.913497E-570.0078351
AAGGGCT150.00222210970.00783569
AGGTGGG550.070.00783570
ATACGCA150.00222210970.0078354
ATTAAAC207.913497E-570.0078351
TTGACAA150.00222210970.00783558
TTGATAA207.913497E-570.00783558
AAAACCC150.00222210970.0078353
CTTGATA207.913497E-570.00783557
ACTGGGG150.00222210970.00783570
TCTTTAT2850.070.0078351
TTTACAC550.070.0078352
Produced by FastQC (version 0.11.5)