FastQCFastQC Report
Thu 26 May 2016
SRR1781397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231408
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16948873.24206596141879No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG27711.1974521192007193No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4550.19662241581967782No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3760.16248357878725023No Hit
TCTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGT3320.14346954297172093No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3130.1352589365968333No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.13482679941920764No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3030.13093756482057664No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2810.12143054691281199No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.11710917513655535No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2410.10414505980778539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTGG150.002217319870.042441
TGGGTCT150.002217319870.042439
TGCTCCT150.002219223270.0272533
GTGGACG150.002219223270.0272549
CATCAGT150.002221127770.0121157
GTAACAT150.002221127770.0121158
CGTCATC150.002223033569.996971
CTTATGC207.9169506E-569.996971
CTTGTGC252.8345476E-669.996971
CTTGTAC150.002223033569.996971
ATCTTGC150.002226848669.9667312
GACGGTG150.002226848669.966737
GATCTTG150.002226848669.9667311
ATGCCGC150.002226848669.9667323
ATCGCCG150.002226848669.966735
TCATCGC150.002226848669.966733
TGTACGC150.002226848669.966733
GCCGATG150.002226848669.9667319
TACCAGG207.933923E-569.966737
CCGATGC150.002226848669.9667320
Produced by FastQC (version 0.11.5)