FastQCFastQC Report
Thu 26 May 2016
SRR1781397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231408
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT6399827.655915093687337No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG161816.992411671160894No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT91393.949301666320957No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT71963.109659130194289No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45661.9731383530387887No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC36641.5833506188204383No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT31481.360367835165595No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT30061.2990043559427504No Hit
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT29321.2670262047984513No Hit
TCATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG20910.9035988384152667No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC18340.7925395837654704No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT18330.7921074465878449RNA PCR Primer, Index 43 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT16430.7100013828389684No Hit
TCTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG15760.6810481919380488No Hit
CATAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT13740.5937564820576644No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT10060.4347300006914195No Hit
CAAAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT9820.42435870842840356No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC8550.36947728686994397No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG7800.3370669985480191No Hit
TCAAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7260.31373159095623315No Hit
CTAATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT6530.28218557698955954No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC6370.2752713821475489No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGAATCTCGTATGCCGT6340.2739749706146719No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC5230.22600774389822303No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT5220.22557560672059737No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG4890.21131507985895043No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAACAGCATCTCGTATGCCGT4870.21045080550369907No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT4520.19532600428680078No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCCGCATCTCGTATGCCGT4130.17847265435939985No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT4050.17501555693839452No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT3940.1702620479845122RNA PCR Primer, Index 43 (95% over 21bp)
CTCTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC3740.1616193044319989No Hit
CTCATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC3620.1564336583004909No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG3310.14303740579409527No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT3100.1339625250639563No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT3000.12964115328769965No Hit
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG2930.12661619304431998No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGACGT2800.12099840973518634No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGAATGCCGT2670.1153806264260527No Hit
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC2630.11365207771555004No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT2610.11278780336029869No Hit
ATACCCATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT2520.10889856876166772RNA PCR Primer, Index 43 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGA2450.10587360851828805No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTAGTATGCCGT2430.10500933416303672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.00222265370.0000131
AAACTGC150.00222265370.0000151
CGGCGCA150.00222265370.0000116
TCCGATA150.00222265370.0000113
ACCATAA150.00222265370.0000131
TCTTCTC150.00222265370.0000150
ATAATCT150.00222265370.0000134
CGCATCA150.00222265370.0000134
CACGCAA150.00222265370.0000132
ATTGTGG150.00222265370.0000129
GCTGATT150.00222265370.0000125
GAATACC150.00222265370.0000142
TGCGGCA150.00222265370.000018
CCGTCGT150.00222265370.0000147
GACCATA150.00222265370.0000130
TCAATAT301.0206713E-770.000011
AATGAAG150.00222265370.0000143
TAACAAG150.00222265370.0000143
CGAATCT150.00222265370.0000134
GAAAACG150.00222265370.0000118
Produced by FastQC (version 0.11.5)