FastQCFastQC Report
Thu 26 May 2016
SRR1781396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230784
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17293774.934570854132No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG19780.8570784803105934No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4690.20322032723239045No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3740.16205629506378258No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630.15728993344425957No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.15252357182473655No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3420.14819051580698836No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3030.13129159733777038No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2590.11222615085967833No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2460.10659317803660565No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2310.10009359400998336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCG207.899553E-570.0279841
TAAGTTC150.00221912470.0279837
CTTGGGC207.899553E-570.027981
CTTGTGC150.00221912470.027981
ATATCGG207.899553E-570.0279842
GGTATCA252.8307204E-670.01279456
TGTGTAG252.8307204E-670.01279435
GTGTTGA150.002221033770.0127936
CATGAAA150.002221033770.0127961
GGGTAGC150.002221033770.0127955
GATGTGT150.002221033770.0127933
AGGGTAG150.002221033770.0127935
ATGTGTA150.002221033770.0127934
GGGAGCA150.002221033770.0127956
GCAGTAA150.002221033770.0127960
GAGGGTA150.002221033770.0127934
GTCTGGC600.069.98244510
GTATACA150.002224857269.9824452
CTACCCG150.002224857269.98244526
CTCGAGC150.002224857269.98244513
Produced by FastQC (version 0.11.5)