FastQCFastQC Report
Thu 26 May 2016
SRR1781396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230784
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT7130730.897722545757073No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG177447.688574597892401No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT110824.801892678868552No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT84083.6432334997226845No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC40341.7479547975596228No Hit
CTAAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT39301.7028910149750414No Hit
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT37741.6352953410981697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35541.5399681087077095No Hit
TCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG25301.096263172490294No Hit
CAAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT22000.9532723239046035No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT21910.9493725734886301RNA PCR Primer, Index 24 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG19320.8371464226289517No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC19050.8254471713810316No Hit
CATAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT18480.8007487520798668No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT13910.6027280920687742No Hit
CAAAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT13070.5663304215196894No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC9580.4151067665002773No Hit
TCAAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG9090.39387479201331116No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT7710.33407861896838603No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT6870.29768094841930115No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC6220.2695160843039379No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC5190.22488560732113147No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG5100.2209858569051581No Hit
CTCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC4920.2131863560732113No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT4810.20841999445368828No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC3960.1715890183028286No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG3670.15902315585135884No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT3500.15165696062118691No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT3380.14645729339988908No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC3380.14645729339988908No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG2990.1295583749306711No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTAGTATCTCGTATGCCGT2360.10226012201885745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGGG353.6907295E-970.0123670
TCATTAT353.6907295E-970.012361
CTCATAA353.6907295E-970.012361
AGGGGGT207.9082965E-570.0123570
ATGGGCG150.002221090370.0123570
TCCTATA207.9082965E-570.012351
AGGGTGG252.8308277E-670.0123570
CTCTATA252.8308277E-670.012351
TCTATAT150.002221090370.012351
AGGGAGG207.9082965E-570.0123570
ATTAAAC252.8308277E-670.012351
CTAAACA455.456968E-1270.012351
AAAAATT150.002221090370.0123566
TCTTTAT1750.070.012351
TGAAAAT207.9082965E-570.0123559
TCTTAAA150.002221090370.012351
CGACACG150.002223000469.99718523
TCCTTGA252.8344766E-669.99718555
ACACGAC150.002223000469.99718525
AAACTCT150.002223000469.99718536
Produced by FastQC (version 0.11.5)