FastQCFastQC Report
Thu 26 May 2016
SRR1781395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306257
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT10046332.80349510378539No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG216217.059757001472619No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT151504.94682570520837No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT116593.806933392542864No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC54911.7929386103827831No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT52791.7237157028247518No Hit
CATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT49411.613350878510532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45171.4749050633944694No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC32121.0487923541339463No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT29740.9710798447055904RNA PCR Primer, Index 45 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG29710.9701002752590145No Hit
CAAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT29380.9593250113466795No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG23180.7568806590543237No Hit
CATAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT22360.7301057608479153No Hit
CAAAAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT16580.5413753808076224No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT14170.46268330193269047No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC13540.4421123435545963No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT12420.4055417508824288No Hit
TCAAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG11310.36929768135911994No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTATCTCGTATGCCGTC10270.3353392738778216No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC9210.3007278200988059No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATATTAATCTCGTATGCCGT9070.29615649601478494No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG9050.2955034497170677No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC9040.29517692656820904No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT7790.25436153296087927No Hit
CTCATATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC6580.21485223194898403No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG5970.1949343198686071No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC5780.18873038004029297No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATAATCTCGTATGCCGTC5410.17664902353252335RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCTTATAAATCTCGTATGCCGT5340.17436336149051287No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG5250.17142465315078512No Hit
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC4540.1482415095818218No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATATCGTATGCCGT4050.13224187528774853No Hit
CTTATACACATCTCCGAGCCCACGAGACACTTATTAATCTCGTATGCCGT3840.12538488916171714No Hit
CTAATACACATCTCCGAGCCCACGAGACTCTTAATAATCTCGTATGCCGT3370.11003830116536112No Hit
ATACCCATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT3270.10677306967677473RNA PCR Primer, Index 45 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTCTAATTAATCTCGTATGCCGT3160.10318131503932972No Hit
TCTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT3130.1022017455927538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTG353.6889105E-970.0285970
AGGGGGT353.6889105E-970.0285970
AGGGTGG353.6889105E-970.0285970
AGGGAGG252.8291452E-670.0285970
ATGGGTG207.903345E-570.0285870
ACAAAGG207.903345E-570.0285866
AACGCCG150.00221974470.0285867
AGTGGGG600.070.0285870
AGGGCGG650.070.0285870
AATGGGT207.903345E-570.0285869
TCCTTAT353.6998244E-970.0057141
TAAACCA150.00222262270.0057142
GAAATAA150.00222262270.00571460
GATACAC252.8346403E-670.0057143
TGCAAAA150.00222262270.00571459
ACCACAT401.3460522E-1070.0057145
TCAATAT301.02119884E-770.0057141
TAACCAC207.916145E-570.0057143
GAACAAA207.916145E-570.00571460
CTAAACC150.00222262270.0057141
Produced by FastQC (version 0.11.5)