FastQCFastQC Report
Thu 26 May 2016
SRR1781394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545513
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15464428.34836200053894No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG15320.28083657034754445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2400.052.51148656
CCGATTT200.006954157752.4922330
CGCCGTA2300.051.7504553
CCGTATC2500.047.61041355
ACGCTAG305.867681E-446.6811635
TCGCCGT2400.046.67687652
TTGTGCG451.6869344E-646.6554832
ACGTTGA305.886277E-446.65120716
CGGCCAA305.886277E-446.65120718
CTCGGGT305.888938E-446.6469312
GTCGCCG2650.046.23652651
GCCGTAT2500.046.21010654
GTAGATC3050.045.9285338
TCGGTGG3050.045.91589445
GGTCGCC2700.045.37613750
ATCTCGG3100.045.1877542
GTGGTCG2750.044.55520248
GATCTCG3200.043.77563541
TGGTCGC2650.043.5944449
TCTCGGT2900.043.46974643
Produced by FastQC (version 0.11.5)