FastQCFastQC Report
Thu 26 May 2016
SRR1781394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545513
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT6117111.213481621886189No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG160722.946217597014187No Hit
CTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT92321.692351969613923No Hit
CTTAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT64541.1831065437487283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC32740.6001690152205356No Hit
CATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT30890.5662559829004992No Hit
CTAAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT29990.5497577509610221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27900.5114451901237917No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT24630.4515016140770247Illumina PCR Primer Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC24360.4465521444951816No Hit
TCATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG23170.4247378155974285No Hit
CAAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT18790.3444464201586397No Hit
TCTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG15810.28981894107014866No Hit
CATAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT13510.2476567927803737No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC11070.202928252855569No Hit
CTTATACACATCTCCGAGCCCACGAGACTGAGCCTTATCTCGTATGCCGT10440.19137949049793496No Hit
CAAAAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT9350.17139829848234597No Hit
TCAAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG8130.1490340285199436No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC6750.12373673954607864No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC6580.12062040684639962No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT6170.1131045456295267No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTGCATTATCTCGTATGCCGT5600.10265566540119117No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5490.10063921483081063No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGT150.002224056570.0017569
CTTACGA150.002224865169.9953214
AGGGGGG124450.064.9112270
CTAAAAC10050.057.4693871
AACTCGA553.6379788E-1157.27415537
AACACAT31250.057.014655
CACGTAT2750.056.00139639
ATCGCAT252.3896257E-456.00139212
ACTCGTA4100.055.4891838
CTTAAAC13400.055.3795931
TAACACG700.055.0013736
ACTCGAA653.6379788E-1253.84749638
TAAATCG653.6379788E-1253.84749636
AAGCCGA1700.053.52583744
AAAACAC17800.053.489383
AAACACA37850.053.0841264
CATTATC2650.052.83150533
TCGTAAG5900.052.79308340
TAAAACA11250.052.5839082
AAGCGTA200.006949405752.50130529
Produced by FastQC (version 0.11.5)