FastQCFastQC Report
Thu 26 May 2016
SRR1781393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288524
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21648875.03292620371269No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG22310.7732458998211588No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6330.21939249421191998No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4640.16081851076513567No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4580.1587389610569658No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4410.15284690355048453No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3910.13551732264906904No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3350.11610819203948372No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3300.11437523394934217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCACT150.002217142670.0482444
TTGTTCG150.002217142670.0482439
CAACTAG150.002217142670.0482439
CATCCAC150.002217142670.0482443
CTAGGAA150.002217142670.0482442
CTTGTGC455.456968E-1269.999661
GTTGTGC207.9187565E-569.999661
TCTTTAT207.9187565E-569.999661
GGTCTAG150.002224783269.9875232
AAGTAAC150.002226313669.975398
GACAGGC150.002226313669.975397
TTGTGCG600.069.975392
CGCGGCT150.002226313669.975397
ATGTGAA150.002226313669.9753918
ACTACAT150.002226313669.9753913
CTGCAGA150.002226313669.9753918
CGTACTC150.002226313669.9753923
TCGTACT150.002226313669.9753922
CTACATG150.002226313669.9753914
CACGTCT207.932371E-569.975397
Produced by FastQC (version 0.11.5)