FastQCFastQC Report
Thu 26 May 2016
SRR1781393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288524
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT8762230.369050754876543No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG220657.647544051794651No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT135314.689731183541057No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT104923.6364392563530243No Hit
CTAAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT49181.7045375774632265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC45531.5780316368828935No Hit
CATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT43261.4993553395904673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39421.3662641582675965No Hit
TCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG30371.0525987439519764No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC30081.0425475870291552No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT28981.0044225090460412RNA PCR Primer, Index 32 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT25210.8737574690493685No Hit
TCTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG24570.8515756054955568No Hit
CATAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT21190.734427638601988No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT15020.520580610278521No Hit
CAAAAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT14840.5143419611540114No Hit
TCAAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG11090.38437010439339536No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC10950.379517821740999No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATCTTAATCTCGTATGCCGT10940.3791712301229707No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC10370.35941550789535703No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG8400.29113695914378007No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC8230.2852449016372988No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC7740.2682619123539116No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACTTAATCTCGTATGCCGT7010.24296072423784504No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT6870.2381084415854487No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT5610.19443789771388167No Hit
CTCATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC5440.18854584020740042No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5050.17502876710429635No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG4900.1698298928338717No Hit
CTCTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC4850.16809693474373016No Hit
ATACCCATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT3700.12823889867047455RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCACTCTTAATCTCGTATGCCGT3660.12685253219836132No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT3290.11402864233131385No Hit
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC3220.11160250100511568No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCATAATCTCGTATGCCGT3210.11125590938708739No Hit
TCATTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC3150.10917635967891753No Hit
CTAATACACATCTCCGAGCCCACGAGACCAATCTAAATCTCGTATGCCGT3130.1084831764428609No Hit
TCTATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC3010.10432407702652119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTGT150.002222359270.0067655
GAGTACG150.002222359270.0067629
TCCTCGT150.002222359270.0067654
TTATATA150.002222359270.006762
CAGGGCC150.002222359270.006764
ACGATGG150.002222359270.0067633
TCTACAC150.002222359270.006764
TACGATG150.002222359270.0067632
CTTGCAA150.002222359270.0067657
TCATGTT150.002222359270.006765
CTGCATA150.002222359270.0067654
GATATAA150.002222359270.0067656
TTGAAAC207.914783E-570.0067658
TTAACAC150.002222359270.006762
CATGTTC150.002222359270.006766
AGGGGGT301.0208896E-770.0067670
CTGGGGG150.002222359270.0067670
AGGGCCG150.002222359270.006765
ATGCACA150.002222359270.006764
GTACGAT150.002222359270.0067631
Produced by FastQC (version 0.11.5)