FastQCFastQC Report
Thu 26 May 2016
SRR1781392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421938
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12884330.536002919860262No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG15600.36972256587460717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGG501.4551915E-1162.9714139
CGCGGGT252.3946611E-455.9745867
ACGTGTT407.44747E-752.5259837
GGCGATA200.00693541552.5259840
CGTTTCC200.00695170152.49484330
CCGTTTC200.00695496152.48861729
GACGGAC200.00695822352.48239519
CCGAACA200.00695822352.48239517
GGACACG200.00695822352.48239522
AGCCGTT200.00696148652.47617711
TCTGGCG800.052.47617711
ACGACGT200.00696148652.47617713
CGCCGTA2700.051.86520853
GTGGTCG3000.051.35264648
TCGCCGT2750.050.9282452
GTCGCCG2750.050.9282451
CGGTGGT3250.050.6344346
TCGGTGG3350.050.17407245
ATCTCGG3150.050.02474242
GTCCGAT352.0805724E-550.0188061
Produced by FastQC (version 0.11.5)