FastQCFastQC Report
Thu 26 May 2016
SRR1781392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421938
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT5127612.152496338324587No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG133623.166815977702885No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT76491.8128255810095324No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT59641.413477809535998No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC29180.6915707995013486No Hit
CTAAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT28110.6662116235086671No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27840.659812579099299No Hit
CATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT26870.6368234195545317No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT20290.48087633728178075RNA PCR Primer, Index 45 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG19950.4728182813588726No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC17750.42067791950476136No Hit
CAAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT15920.37730661850793246No Hit
TCTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG13420.3180562073100787No Hit
CATAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT12510.29648905763405997No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCAATTATCTCGTATGCCGT10680.2531177566372311No Hit
CAAAAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT9820.23273561518516941No Hit
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC7780.18438727964772075No Hit
TCAAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG6970.16519014641961616No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTCATATATCTCGTATGCCGT5420.12845489147694683No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC5100.12087083884362157No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG4980.1180268191061246No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC4960.11755281581654177No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT4670.11067976811759074RNA PCR Primer, Index 45 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGTCATTAATCTCGTATGCCGT4310.10214770890509979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG150.002223820770.0008324
GACCGGG150.002223820770.0008357
GACGAGT207.922344E-570.000836
TATCGCC150.002223820770.0008334
GCGAGTA150.002223820770.0008332
GTTTCGG150.002224866369.9925447
AGACGAC150.002224866369.9925445
CCGAATT150.002224866369.9925447
GCTCGTT150.002224866369.9925438
AGGGGGG105050.065.1518670
CGACTTC3200.059.05620248
ACGGTAA1200.058.33402627
ATCGAAA308.376232E-658.32711439
CTTAAAC11650.058.290861
CCGACTT2650.056.78639647
CTAAAAC9250.056.7641561
CGGTCAA5550.056.76415328
AACACAT29450.056.689985
AGCCGAC509.804353E-1055.9940345
TAAATCG509.804353E-1055.9940336
Produced by FastQC (version 0.11.5)