FastQCFastQC Report
Thu 26 May 2016
SRR1781391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187686
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14086775.05461249107552No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG13440.7160896390780346No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4280.22804045053973127No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630.19340813912598703No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3410.18168643372441204No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2930.15611180375733938No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2900.15451338938439735No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2780.14811973189262917No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700.14385729356478374No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG2090.11135620131496222No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2000.10656095819613609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCTG150.00221444270.0600838
CCCAGTC150.00221444270.0600836
CCAGTCT150.00221444270.0600837
GCGGTAG150.00221444270.0600860
ATCAGTA150.00221444270.0600859
AGTGTAG252.817318E-670.0600835
TGGTGGC150.00221444270.0600850
CCCCAGT150.00221444270.0600835
GGGTGTA252.817318E-670.0600834
CAGTGTA150.00221444270.0600834
GGCGGTA150.00221444270.0600859
AGTAAAA207.8882105E-570.0413862
TAAGAAG150.00221678970.04137464
CATTAAG150.00221678970.04137461
ATTAAGA150.00221678970.04137462
CGGGAGA150.00221678970.04137461
TTAAGAA150.00221678970.04137463
GCCCCCA150.00221678970.04137433
GTGCCCC150.002221488270.00400531
TCGTGCC150.002223840769.9853329
Produced by FastQC (version 0.11.5)