FastQCFastQC Report
Thu 26 May 2016
SRR1781391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187686
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT3943721.012222541905096No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2089011.130292083586415No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT89874.788316656543375No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT82254.382319405816097No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG42732.2766748718604477No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT38542.0534296644395424No Hit
TCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG35421.8871945696535701No Hit
CATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT34531.8397749432562898No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT25121.3384056349434694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21231.1311445712519848No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC20781.107168355657854No Hit
TCAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG18791.0011402022526987No Hit
CATAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT14860.7917479193972912No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT14630.7794934092047355TruSeq Adapter, Index 18 (95% over 21bp)
ACAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9600.5114925993414533No Hit
TCTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG8570.45661370587044314No Hit
CTCATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC8500.45288407233357847No Hit
ACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6450.34365909018253893No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT5910.31488763146958215No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC5750.3063627548138913No Hit
ACTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG4030.21472033076521424No Hit
ACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG3390.18062082414245068No Hit
CTTATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT3390.18062082414245068No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3220.17156314269577913No Hit
CTCAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC3010.16037424208518483No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGACGT2960.15771021813028144No Hit
CTAATACACAACTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2870.1529149750114553No Hit
TCATTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC2810.1497181462655712No Hit
CTAATACACATCTCCGAGCCAACGAGACTACACCTCATCTCGTATGCCGT2770.1475869271016485No Hit
CTAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG2570.1369308312820349No Hit
CTAATACACATCTCCGAGACCACGAGACTACACCTCATCTCGTATGCCGT2550.13586522170007354No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATATCGTATGCCGT2540.13533241690909284No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT2510.1337340025361508No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGAATGCCGT2510.1337340025361508No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTAAGCCGT2380.12680754025340196No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT2340.12467632108947924No Hit
CTAATACACATCACCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2330.12414351629849854No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACACATCATCTCGTATGCCGT2210.11774985880673039No Hit
CTAAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC2190.11668424922476903No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCACATCTCGTATGCCGT2180.11615144443378836No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCAACTCGTATGCCGT2130.11348742047888494No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC2130.11348742047888494No Hit
TTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC2130.11348742047888494No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCAGT2050.10922498215103951No Hit
CAAATACACAACTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT2030.10815937256907814No Hit
CTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGA1990.10602815340515542No Hit
CAAAAACACATCACCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT1970.10496254382319406No Hit
CAAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTAAGCCGT1950.10389693424123271No Hit
CACATATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC1930.10283132465927133No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC1890.10070010549534862No Hit
CTAAAACACATCACCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT1880.10016730070436793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGA150.002216028370.0474670
AGGGGCG150.002216028370.0474670
AGTGGGG301.0148324E-770.0474670
TGGGGGG455.456968E-1270.0474670
TGAAAAC252.8248196E-670.028859
GAAAAGG150.002218373870.0287966
CATTTTG150.002218373870.0287960
CTCTATA150.002218373870.028791
TCTATAT150.002218373870.028791
CTCTAAT550.070.028791
ACTTAAT150.002218373870.028791
AAGAAGG301.0167241E-770.0287966
TCATTAT401.3460522E-1070.028791
TCTTTAT301.0167241E-770.028791
CTCAATA207.8952595E-570.028791
GAAGGGG301.0167241E-770.0287968
TCAATAC207.9056954E-570.010132
TACACGT150.00222072170.010135
TCTATAC150.00222072170.010132
CATTATA401.3460522E-1070.010132
Produced by FastQC (version 0.11.5)