FastQCFastQC Report
Thu 26 May 2016
SRR1781390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155125
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11538374.38066075745367No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG9410.6066075745366639No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2390.15406929895245772No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2360.15213537469782434No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2230.14375503626107977No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1720.11087832393231266No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG1660.10701047542304593No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600.1031426269137792No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.10185334407735697No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.10185334407735697No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1560.10056406124093473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCC150.002219396470.0148436
GTTCCGC150.002219396470.0148435
TCGCGTT150.002219396470.0148431
ACACCTG150.002219396470.0148433
CGTAGGC150.002219396470.0148436
TACACCT150.002219396470.0148432
CAGGGAC150.002219396470.0148454
GGAAGTT150.002219396470.0148431
CGCGGTA150.002219396470.0148453
AGTTGCC150.002219396470.0148447
AGCATAT150.002219396470.0148460
TCCGCCA150.002219396470.0148437
GCCGAAA150.002219396470.0148455
AGTTCCG150.002219396470.0148434
CGCGTTC150.002219396470.0148432
AAGTTCC150.002219396470.0148433
GTCTATC150.00222508569.969691
GACCAAT150.00222508569.9696922
GCCTCGG150.002227933469.947137
CATCGCC150.002227933469.947133
Produced by FastQC (version 0.11.5)