FastQCFastQC Report
Thu 26 May 2016
SRR1781390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155125
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT4306427.760838033843676No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG111137.163900080580177No Hit
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT62074.001289282836423RNA PCR Primer, Index 47 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT48773.143916196615633No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT23381.5071716357775986RNA PCR Primer, Index 47 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT22641.459468170829976RNA PCR Primer, Index 47 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT21991.417566478646253RNA PCR Primer, Index 47 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT21561.3898468976631748Illumina PCR Primer Index 9 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19431.2525382755842063No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC19191.2370668815471395No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC18701.2054794520547945No Hit
TCATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG15390.9921031426269138No Hit
CAAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT13290.8567284448025786No Hit
CATAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT11130.7174858984689767RNA PCR Primer, Index 47 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG10020.6459307010475422No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC8430.5434327155519743RNA PCR Primer, Index 47 (95% over 22bp)
CAAAAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT8040.5182917002417405No Hit
CTAATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT6030.3887187751813054No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC5740.37002417405318294No Hit
TCAAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5560.35842062852538276No Hit
TTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC5160.33263497179693796RNA PCR Primer, Index 47 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG5040.3248992747784045No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT4650.2997582594681708No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCG4120.26559226430298144No Hit
CTTAAACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT4080.263013698630137RNA PCR Primer, Index 47 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT3740.2410958904109589No Hit
TCTTATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCG3740.2410958904109589No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG3690.2378726833199033No Hit
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC3510.22626913779210314No Hit
TCTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT2800.18049959709911362No Hit
CATATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT2490.1605157131345689No Hit
CTAAAACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT2390.15406929895245772No Hit
ATACCCATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT2340.15084609186140208Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCACGCAGATCTCGTATGCCGT2240.1443996776792909No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG1950.12570507655116842No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCTCGCAGATCTCGTATGCCGT1810.11668009669621272No Hit
CTAATACACATCTCCGAGCCCACGAGACCCTCGCAGCTCTCGTATGCCGT1710.11023368251410154No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTCGAAGATCTCGTATGCCGT1690.10894439967767929RNA PCR Primer, Index 47 (95% over 23bp)
CTCATATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC1670.10765511684125706No Hit
TCATTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC1660.10701047542304593No Hit
CAAATACACATCTCCGAGCCCACGAGACACTCGCAGATCTCGTATGCCGT1620.10443190975020145RNA PCR Primer, Index 47 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAGGG207.8888275E-570.03258567
AGGGGTG150.002217169470.0325870
TCGGCTT150.002217169470.0325867
ATTGGTC150.002217169470.0325862
GATTGGT150.002217169470.0325861
AGGGAGG150.002217169470.0325870
AGGTGGG252.8215982E-670.0325870
GGCTTGC150.002217169470.0325869
CGGCTTG150.002217169470.0325868
TTGGTCG150.002217169470.0325863
CCGATTG150.002217169470.0325859
GTCGGCT150.002217169470.0325866
ACACTAG207.901447E-570.0127
GACACTA207.901447E-570.0126
AGAATGA207.901447E-570.0155
GAACCAC207.901447E-570.0126
CACTAGC207.901447E-570.0128
ACTAGCA207.901447E-570.0129
TTACTGT150.002220008570.00999557
ATAACTC150.002220008570.00999535
Produced by FastQC (version 0.11.5)