FastQCFastQC Report
Thu 26 May 2016
SRR1781389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192291
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18979015.918093821055429No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG19170.16078289612183602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA200.00696803452.4696165
TTATGCG506.2198524E-848.981912
CGTATCA4700.046.18610456
CGTCTGG1450.045.8374529
CGCCGTA4750.045.70185553
CCGTATC4700.045.4449855
TTGTGCG703.8562575E-1044.983392
TATGCGC551.3182216E-744.521543
GTGGTCG5600.043.14708748
TCGCCGT5050.042.98689352
ATCTCGG5300.042.29289642
TCTCGGT5300.042.29289643
CGGTGGT6400.041.5872846
GATCTCG5500.041.39177341
TGCCGGC2700.040.16530219
GGTCGCC5800.039.2376450
CTCGGTG6100.039.04129444
GTCGCCG5850.038.9055451
GCCGTAT5700.038.6991554
TCGGTGG6900.038.06616245
Produced by FastQC (version 0.11.5)