FastQCFastQC Report
Thu 26 May 2016
SRR1781389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1192291
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT735006.164602433466326No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG176881.4835304468456105No Hit
CTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT115450.9683038788349487No Hit
CTTAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT88630.7433587941198918No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC42660.3577985575669027No Hit
CTAAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT41440.34756615624876813No Hit
CATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT37850.31745605728802784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35160.2948944511029606No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT26110.21899016263647048Illumina PCR Primer Index 6 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG25540.21420945054521087No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC24230.203222199949509No Hit
CAAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT23960.20095765211680705No Hit
CATAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT19730.1654797360711437No Hit
TCTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG19630.16464101465162448No Hit
CAAAAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT14660.12295656010151883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG152300.064.3754170
TAACTCG5950.056.46852536
ACTCGTA5550.055.4934738
CCGAATT700.054.99568647
ATGCCGA5650.053.2723944
CCGACTT4600.053.2566947
GCCGTTT200.00694849452.5068959
CGTTATG200.006949646452.50468466
TCTAGCG200.006953105352.4980835
ATCGTTA200.006953105352.4980832
GCGTACC200.006953105352.4980815
TACTGCG200.00695425952.4958848
TCGTAAG7950.052.38802340
TGCCGAC5350.051.6803545
TATGCCG190300.051.0175843
CCGTCTT179950.050.7990147
GCCGTCT182600.050.77093546
TGCCGTC187000.050.6639245
GTATGCC192350.050.63760842
ATGCCGT189450.050.6368444
Produced by FastQC (version 0.11.5)