FastQCFastQC Report
Thu 26 May 2016
SRR1781388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239708
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18182875.85395564603601No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG20350.8489495552922723No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5030.20983863700836017No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4550.1898142740334073No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260.17771622140270663No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4090.17062425951574414No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3300.1376674954528009No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.12765531396532448No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2950.12306639745023112No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.11305421596275468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAT150.00222061870.01691454
AGTAGAT150.00222061870.01691437
TCAGGAA150.00222061870.01691460
GCGGCTT150.00222061870.01691456
GGCGAGT150.00222061870.01691433
CGGATCA207.906354E-570.01691456
ATCAGGA150.00222061870.01691459
GTGGGTC150.00222061870.01691438
GGCTTTG150.00222061870.01691458
TATCATA150.00222061870.01691458
CGAGTAG150.00222061870.01691435
TGGGTCT150.00222061870.01691439
GCGAGTA150.00222061870.01691434
CTGCGGA207.92273E-569.98768618
TTCTCCG150.002224369.98768619
TGCGGAG150.002224369.98768619
GACGACC252.8370905E-669.9876823
ACGTCGG150.002226142769.9730768
ACATATG150.002226142769.9730768
CTTATGC207.930928E-569.9730761
Produced by FastQC (version 0.11.5)