FastQCFastQC Report
Thu 26 May 2016
SRR1781388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239708
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT7283130.383216246433157No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG169247.060256645585462No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT113114.718657700201912No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT88623.6969980142506715No Hit
CTAAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT41671.738365010763095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC39491.6474210289185176No Hit
CATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT38711.6148814390842192No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36551.5247718056969313No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT24681.0295859962954927RNA PCR Primer, Index 20 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG24651.0283344736095583No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC23300.9720159527425033No Hit
CAAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT23200.9678442104560548No Hit
CATAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT19050.7947169055684415No Hit
TCTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG18820.78512189830961No Hit
CAAAAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT13520.5640195571278389No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC11200.4672351360822334No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAAAGCCATCTCGTATGCCGT9000.3754568057803661No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGACATCTCGTATGCCGT8870.370033540807983No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCAATCTCGTATGCCGT8850.3691991923506934No Hit
TCAAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG8810.36753049543611394No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC7890.3291504664007876No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC7240.3020341415388723No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6140.25614497638793865No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT5660.23612061341298582No Hit
CTTATACACATCTCCGAGCCCACGAGACGAACTGCCATCTCGTATGCCGT5150.2148447277520984No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4470.18647688020424852No Hit
CTCATATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC4450.1856425317469588No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG4140.17271013065896842No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC3780.15769185842775377No Hit
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC3680.15352011614130526No Hit
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT2790.11639160979191349No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGACGT2710.11305421596275468No Hit
ATACCCATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT2660.11096834481953043RNA PCR Primer, Index 20 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT2640.11013399636224074No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGA2550.10637942830443706No Hit
CTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTAAGCCGT2540.10596225407579223No Hit
TCATTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC2480.10345920870392311No Hit
CTAATACACAACTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT2470.10304203447527825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAAGG150.002215688870.0561766
AGGGGTG301.0166332E-770.0415470
AGGGGGC150.002217524770.0415470
AGGGGAG150.002217524770.0415470
AAGGGGT252.8241575E-670.0415469
AGGTGGG650.070.0415470
TAAAGGG150.002217524770.0415467
GCATTAA252.8276627E-670.0269356
TGCATCA207.908945E-570.0123155
AGAACAG150.002221200170.0123128
TGCTTCA401.3460522E-1070.0123155
CTCGGTC150.002221200170.0123129
ATCATGA150.002221200170.0123155
CTAAAAG150.002221200170.0123128
CGAAAAC150.002221200170.0123128
CGGTCAA150.002221200170.0123131
CGCAAAG207.908945E-570.0123128
CAAAATG207.908945E-570.0123128
CTTTACA401.3460522E-1069.99771
TAAAAAC150.002223039569.99773
Produced by FastQC (version 0.11.5)