FastQCFastQC Report
Thu 26 May 2016
SRR1781387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721819
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25234234.959179517302815No Hit
CTTATACACATCTGACGCTGCCGACGAACGTGGGCGTGTAGATCTCGGTG25450.352581464328315No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8170.11318626968810741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA4200.057.5178356
GACGGCT252.3875968E-456.0134833
CGCCGTA4350.055.54601353
CCGTATC4200.055.0246955
TCGCCGT4400.054.9148152
GTGGTCG5050.054.0874248
GGTCGCC4800.053.25675250
CGGTGGT5300.052.86123346
TCTCGGT4800.052.5381443
GTACGCG200.00696916852.4653434
TCGGTGG5700.052.2236545
GCCGTAT4500.052.134554
TGGTCGC5000.051.82684749
TTGTGCG1050.049.973922
CGTCTGG1750.049.973919
CTCGGTG5150.049.640844
TCTGGCG2600.048.43290311
GTCGCCG5350.047.78175751
ATCTCGG5150.046.9272742
GATCTCG5150.046.9272741
Produced by FastQC (version 0.11.5)