FastQCFastQC Report
Thu 26 May 2016
SRR1781387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721819
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT10530414.588698828930799No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG221033.0621249925535348No Hit
CTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT161522.2376800832341623No Hit
CTTAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT127631.7681717993014867No Hit
CTAAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT59460.8237522148904365No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC54230.7512963776237533No Hit
CATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT54220.7511578387379662No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45270.6271655359584605No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT34580.4790674670519895No Hit
CAAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT32680.45274507875242964No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC32160.4455410566914975No Hit
TCATATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG30800.42669976822444405No Hit
CATAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT26680.3696217472801353No Hit
TCTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG24840.3441305922952984No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGAATTATCTCGTATGCCGT22050.30547824316068156No Hit
CAAAAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT19160.2654405051681931No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC17450.24175035569858924No Hit
TCAAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG12620.17483607386339234No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAATAATCTCGTATGCCGT11520.15959679642680505No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC10510.1456043689623022No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC9950.13784619135822138No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG9560.13244317481252227No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGAAATATCTCGTATGCCGT9120.12634746383788734No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT7680.10639786428453671No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC207.929032E-569.99570537
AGGGGGG207000.065.816470
CTAAAAC17900.059.4459341
CTTAAAC23500.058.3874861
AACACAT55850.058.285615
AAAACAC29400.056.075763
TAAAACA19050.056.041072
TATTACG252.3911636E-455.9965736
AAACACA64150.055.5459824
CACGTAA1650.055.1481339
AATCTCG13450.054.12307436
TAAACAC35900.053.7228133
TTAAACA25750.053.42162
TAACTCG6750.052.88564736
TCAGTCG200.006946970252.50768759
GCCGAAT800.052.49677746
TACCGTT200.006952682552.49677718
ATGCCGT262650.052.3135644
TATGCCG263000.052.3104843
CCGTCTT250700.052.3083247
Produced by FastQC (version 0.11.5)