FastQCFastQC Report
Thu 26 May 2016
SRR1781386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265290
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32556325.730306886168385No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG100160.7915971832544317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG1600.054.652882
CGGTACG200.0069663652.4729923
CGTATCA17300.051.8082756
CCGTATC17200.051.50086255
CGCCGTA17500.050.4179253
GCCGTAT17600.050.1314554
TCGCCGT17550.050.07478352
GTGGTCG19350.049.03939448
GTCGCCG18900.048.9062751
ATCTCGG18400.048.34770242
GGTCGCC19050.048.33930650
CGGTGGT19750.048.053846
TCTCGGT18900.047.9952143
GATCTCG18500.047.89704541
TGGTCGC19300.047.89645849
CTCGGTG19750.047.16906744
TCGGTGG20650.046.13086345
GGTGGTC20850.045.51318447
GTAGATC19800.045.2829538
ACGGGTT404.6090485E-543.7274924
Produced by FastQC (version 0.11.5)