FastQCFastQC Report
Thu 26 May 2016
SRR1781386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265290
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT20274316.02344126642904No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG315582.4941317800662297No Hit
ATTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT176491.3948580957725105No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161101.2732259007816389No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC111890.8843032032182346No Hit
CTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT58240.46028973594986133No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC54830.4333393925503244No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT37430.2958215112740953RNA PCR Primer, Index 17 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT37180.2938456796465633No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT22640.17893131218930047No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC15650.12368705988350497No Hit
AATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT14360.1134917686854397No Hit
TCATATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12950.10234807830615907No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12870.10171581218534882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG280550.066.4396270
CGATTTA252.3928111E-455.9920318
ACTCGTA5150.055.72242738
ATTATAC31350.055.7150151
AAGGGGG334150.055.69840269
TCTCGAA6150.055.19758238
GATCTCG345950.054.3938936
ATCTCGT342750.054.25846537
CCGTCTT339000.054.21647347
TATGCCG345100.054.20121443
GCCGTCT342200.054.179946
TGCCGTC344300.054.17469445
AAGCCGT4850.054.11601344
ATGCCGT344750.054.1141344
AGATCTC347700.054.0999935
ACAGATC350500.054.0850333
CGTCTTC342250.054.04928648
CTCGTAT347750.054.01170339
GTATGCC347400.053.9531842
CTGCTTG341700.053.93358654
Produced by FastQC (version 0.11.5)