FastQCFastQC Report
Thu 26 May 2016
SRR1781385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663811
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34488151.954697948663096No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG112471.6943075664609355No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT13340.2009608156538533No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7770.11705138962746926No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG150.002220870770.0287352
TATGCGT150.002220870770.0287353
ACGGGCT150.002228186769.9706924
TGTTCGA150.002228852869.96541618
TTATGCG150.00222951969.9601442
TATGCGC150.002230851769.949613
CATCGAA252.8518407E-669.949613
CGCGTCT1050.059.9568027
CGTCTGG1350.059.5956769
CGTCTTA308.400773E-658.30451615
CCATCGA308.404526E-658.3001172
CCGTATC16650.056.35520655
TCGCCGA252.3854549E-456.02298752
TTTTACG252.3970334E-455.9681172
CGTATCA16850.055.8898856
CGCCGTA16900.055.7329253
TCGCCGT17200.055.16798852
GCCGTAT17100.055.08107854
CGGTGGT18250.055.0678446
GTGGTCG18150.054.78418748
Produced by FastQC (version 0.11.5)