FastQCFastQC Report
Thu 26 May 2016
SRR1781385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663811
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT20144330.346438971333704No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG303674.574645493973435No Hit
ATTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT164192.4734450016646305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161042.4259917355994403No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC111001.6721627089638467No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT96841.4588489796041344No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC54540.8216194067287224No Hit
CTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT44410.6690157288746345No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT40710.613276971909173RNA PCR Primer, Index 43 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT29310.441541342339913No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC21590.325243179157923No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT17900.26965506748155726No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCG16350.24630504767170175No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG14640.22054470323631276No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACAAGCATCTCGTATGCCGT14500.21843566918897098No Hit
TCATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG10420.1569723912378674No Hit
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC10370.1562191647923882No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC10200.1536581948777589No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC10060.15154916083041708No Hit
AATATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT9930.14959077207217114No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTAAGCCGT8640.1301575297788075No Hit
ATTATACACATCTCCGAGCCCACGAGACAGCCCAGCATCTCGTATGCCGT8570.12910301275513664No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC8320.12533688052774056No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG8220.12383042763678216No Hit
ATAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT7850.11825655194023602No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT7470.112532030954594RNA PCR Primer, Index 43 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG7410.11162815922001895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC150.002225144369.99480425
TAGCGTT150.002225144369.99480416
CCGTGCA150.002225144369.99480425
ACGATCG150.002225144369.99480434
CGCTTCT150.002225144369.99480433
ACTCGTT207.932026E-569.989538
TCGTGGC150.002225809369.98952523
AGGGGGG282300.066.95055470
ATTATAC25950.064.754591
TCGTAAT501.4551915E-1162.9905840
CCCGCAT1950.061.0211132
ACTCGTA3250.060.2986738
AACTCGT3300.059.38505637
AACTCGA650.059.22191237
CTTAAAC5550.058.661971
TCTTTAT7850.058.4209141
CCGTAAA308.379895E-658.32900247
CGCGACA308.383637E-658.32460823
CCGTCTA3600.057.35685347
GCAACTC4350.056.31765435
Produced by FastQC (version 0.11.5)