FastQCFastQC Report
Thu 26 May 2016
SRR1781384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681404
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34093250.033753837664584No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG107811.5821744515735159No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT12240.17962911870197418No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7630.11197468755686787No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC207.939157E-569.9771045
CGCGTCT1050.056.6481327
CCGTATC17700.055.17168855
CGTATCA18000.054.83551456
CGCCGTA18050.054.68361753
GCCGTAT17950.054.59827854
TCGCCGT18200.054.04061552
GTCGCCG19000.053.23896451
GGTCGCC19000.053.05474550
TCTCGGT18650.052.95934743
TGGCGCT2450.052.8398613
GTGGTCG19150.052.8219548
TGGTCGC18900.052.59082849
TTACCGA200.00693159352.53679738
TATCGAA200.006949733552.5020948
TCGGTAG200.006949733552.5020960
TCGAAGA200.006949733552.5020950
ATTGACG200.00695578852.49053231
GCGAACG200.006959826752.48282614
TATGCGC200.006959826752.4828263