FastQCFastQC Report
Thu 26 May 2016
SRR1781384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681404
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT21505831.561012262915977No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG328754.824597448796896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174532.5613292554783946No Hit
ATTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT161002.362768636521065No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC126121.8508843505468122No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC58350.8563201859689699No Hit
CTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT55470.81405451098027No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT44580.654237427429249RNA PCR Primer, Index 24 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT35610.5225974605373611No Hit
CTTAAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT22020.3231563066844339No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC19990.29336487605003786No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTAAGTATCTCGTATGCCGT16690.24493545679215267No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG13390.19650603753426749No Hit
TCATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG13240.19430470029527272No Hit
AATATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT12310.18065640941350505No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGAATCTCGTATGCCGT12200.1790420954382422No Hit
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC10580.15526765325709857No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC10470.15365333928183572No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC10090.1480766182763823No Hit
ATAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT9440.13853749024073822No Hit
CTTATACACATCTCCGAGCCCACGAGACGACATAGTATCTCGTATGCCGT9140.1341348157627487No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC8120.11916572253758417No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG7890.1157903387711255No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT7890.1157903387711255No Hit
CAAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT7640.11212144337280087No Hit
CCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG7120.10449014094428562No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAA150.002224514570.016
CGTTGTC150.002224514570.044
ACGTATC150.002224514570.040
AAGGCGT150.002224514570.031
AGGGGGG290100.066.5856670
TACGAGA501.4551915E-1163.00000413
ATTATAC21400.062.640191
CACGTAT2000.059.50000439
CGACACG308.376446E-658.33333223
CTTAAAC5550.057.387391
AAACTCG1350.057.03703736
AAGGGGG341700.056.43839669
ACTCTCG252.3903437E-456.00000436
GTATCAC2000.056.00000435
ATCACGT1950.055.64102637
TGCCGAC3400.055.58823445
AAGCCGT4100.055.48780444
AAGTATC6550.055.03816633
GAAACTC1400.055.035
ACTCGAA700.055.038
Produced by FastQC (version 0.11.5)